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Anti-Hexokinase II Antibody (1M510) 是一种 Mouse 抗体,靶向 Hexokinase II。Anti-Hexokinase II Antibody (1M510) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Hexokinase II Antibody (1M510) 是一种 Mouse 抗体,靶向 Hexokinase II。Anti-Hexokinase II Antibody (1M510) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 |
| 产品描述 | Anti-Hexokinase II Antibody (1M510) is a Mouse antibody targeting Hexokinase II. Anti-Hexokinase II Antibody (1M510) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG1, k |
| 克隆号 | 1M510 |
| 反应种属 | Human |
| 应用 | WBIHC-PIHC-FrIF |
| 推荐剂量 | WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. |
| 组织特异性 | Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the “set point” for insulin secretion. |
| 免疫原 | Recombinant Protein: human HK2 (Hexokinase II) between 1-170 amino acids |
| 抗原种属 | Human |
| 基因名称 | HK2 |
| 基因ID | |
| 蛋白名称 | Hexokinase-2 |
| Uniprot ID | |
| 功能 | Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. |
| 分子量 | Theoretical: 101 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | store at low temperature | -20°C for 1 year |