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Anti-Her2/ERBB2 Antibody (3Y105)
还可以产品编号 TMAY-00040
别名 erb-b2 receptor tyrosine kinase 2, EGFR2
Anti-Her2/ERBB2 Antibody (3Y105) 是一种 Rabbit 抗体，靶向 Her2/ERBB2。Anti-Her2/ERBB2 Antibody (3Y105) 可用于 ELISA,IHC-P,FCM,ICC/IF。
Anti-Her2/ERBB2 Antibody (3Y105)
Anti-Her2/ERBB2 Antibody (3Y105)
还可以产品编号 TMAY-00040 别名 erb-b2 receptor tyrosine kinase 2, EGFR2
Anti-Her2/ERBB2 Antibody (3Y105) 是一种 Rabbit 抗体，靶向 Her2/ERBB2。Anti-Her2/ERBB2 Antibody (3Y105) 可用于 ELISA,IHC-P,FCM,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μL | ¥ 795 | 5日内发货 | |
| 100 μL | ¥ 2,279 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Her2/ERBB2 Antibody (3Y105) is a Rabbit antibody targeting Her2/ERBB2. Anti-Her2/ERBB2 Antibody (3Y105) can be used in ELISA,IHC-P,FCM,ICC/IF. |
| 别名 | erb-b2 receptor tyrosine kinase 2, EGFR2 |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 3Y105 |
| 交叉反应 | Human |
| 验证活性 | 1. Flow cytometric Analysis of anti-ErbB2 (0.5 μL/test) on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with purified rabbit anti-human ErbB2. Second step staining with FITC conjugated goat anti-rabbit IgG (H+L) polyclonal antibody. 2. Immunochemical staining of human ERBB2 in human breast carcinoma with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 3. Immunofluorescence staining of Erbb2 in SKBR3 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human Erbb2 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and Cell membrane. |
| 应用 | ELISA,IHC-P,FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human HER2/ErbB2/CD340 (rh HER2/ErbB2/CD340; TMPY-01137; NP_004439.2; Met1-Thr652). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 其他可选修饰符 | Biotin |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Human HER2/ErbB2/CD340 Protein (TMPY-01137) |
| 抗原种属 | Human |
| 研究领域 | Cancer Drug Targets, Receptor Tyrosine Kinases (RTKs) |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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