Anti-HDAC8 Antibody (7B830) is a Rabbit antibody targeting HDAC8. Anti-HDAC8 Antibody (7B830) can be used in WB,ICC/IF,FCM.

IgG

7B830

Human

1. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-HDAC8 antibody (TMAB-06945): 1 μg/10^6 cells; Secondary Antibody: Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (HDAC8) monoclonal Antibody, unconjugated (TMAB-06945) 1: 200, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. 25 μg total protein per lane of various lysates (see on figure) probed with HDAC8 monoclonal antibody, unconjugated (TMAB-06945) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human HDAC8

Human

HDAC8

Histone deacetylase 8

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.