Anti-HBsAg Polyclonal Antibody is a Goat antibody targeting HBsAg. Anti-HBsAg Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human,HBV (predicted:Bee,Hepatitis B virus)

1. Paraformaldehyde-fixed, paraffin embedded (Hepatitis B virus infected human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HBSAg) Polyclonal Antibody, Unconjugated (TMAB-00835) at 1:200 overnight at 4°C, followed by A594 conjugated Rabbit Anti-Goat IgG H&L secondary antibody incubation and staining.
2. Measured by its binding ability in a indirect ELISA. Immobilized recombinant HBSAg protein at 2 μg/mL (100 μL/well) can bind  Goat Anti-Human HBSAg Antibody, the EC50 is 29.14 ng/mL.
3. Sample:
Lane 1: Recombinant HBSAg protein
Primary: Anti-HBSAg (TMAB-00835) at 1/1000 dilution
Secondary: IRDye800CW Donkey anti-Goat IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 20 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

Recombinant Protein: HBsAg protein

Hepatitis B