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Anti-HA Tag Antibody (5Z189)

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产品编号 TMAY-00030

Anti-HA Tag Antibody (5Z189) 是一种 Mouse 抗体,靶向 HA Tag。Anti-HA Tag Antibody (5Z189) 可用于 IP。

Anti-HA Tag Antibody (5Z189)

Anti-HA Tag Antibody (5Z189)

Rating icon 还可以
产品编号 TMAY-00030

Anti-HA Tag Antibody (5Z189) 是一种 Mouse 抗体,靶向 HA Tag。Anti-HA Tag Antibody (5Z189) 可用于 IP。

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产品介绍

生物活性
产品描述
Anti-HA Tag Antibody (5Z189) is a Mouse antibody targeting HA Tag. Anti-HA Tag Antibody (5Z189) can be used in IP.
Ig Type
Monoclonal Mouse IgG1
克隆号
5Z189
交叉反应
other
验证活性
HA tag was immunoprecipitated using:

-Lane A:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate.

-Lane B:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate.

-2 µL anti-HA tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-HA tag mouse monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 39 kDa.

-Observed band size: 39 kDa
应用
IP
推荐剂量
IP: 4-8 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Mouse
构建方式This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景The human influenza hemagglutinin (HA) epitope was derived from the protein on the HA virus. HA tag is the amino acid sequence of a YPYDVPDYA tag and a particularly common tags. HA is tagged onto proteins for study and analysis and often used in fusion protein-related research through the realization of detection of HA-tag for the detection of fusion proteins. Antibodies to HA-tag are the key of the most important tools in the detection and often used in purification of HA tag-related proteins, study of the protein interaction and detection of target protein expression.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA).
抗原种属
other
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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4个月前
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