购物车
- 全部删除
- 您的购物车当前为空
Anti-HA Tag Antibody (5Z189) 是一种 Mouse 抗体,靶向 HA Tag。Anti-HA Tag Antibody (5Z189) 可用于 IP。
Anti-HA Tag Antibody (5Z189) 是一种 Mouse 抗体,靶向 HA Tag。Anti-HA Tag Antibody (5Z189) 可用于 IP。
产品描述 | Anti-HA Tag Antibody (5Z189) is a Mouse antibody targeting HA Tag. Anti-HA Tag Antibody (5Z189) can be used in IP. |
Ig Type | Monoclonal Mouse IgG1 |
克隆号 | 5Z189 |
交叉反应 | other |
验证活性 | HA tag was immunoprecipitated using: -Lane A:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate. -Lane B:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate. -2 µL anti-HA tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-HA tag mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 39 kDa. -Observed band size: 39 kDa |
应用 | IP |
推荐剂量 | IP: 4-8 μL/mg of lysate |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | The human influenza hemagglutinin (HA) epitope was derived from the protein on the HA virus. HA tag is the amino acid sequence of a YPYDVPDYA tag and a particularly common tags. HA is tagged onto proteins for study and analysis and often used in fusion protein-related research through the realization of detection of HA-tag for the detection of fusion proteins. Antibodies to HA-tag are the key of the most important tools in the detection and often used in purification of HA tag-related proteins, study of the protein interaction and detection of target protein expression. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA). |
抗原种属 | other |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
评论内容