Anti-GTP cyclohydrolase 1 Polyclonal Antibody is a Rabbit antibody targeting GTP cyclohydrolase 1. Anti-GTP cyclohydrolase 1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Mouse (predicted:Human,Rat)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GTP cyclohydrolase 1) Polyclonal Antibody, Unconjugated (TMAB-00823) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-GTP cyclohydrolase 1 (TMAB-00823) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human GTP-CH-1

Human

GCH1

GTP cyclohydrolase 1

Oxidative Stress,Amino acid metabolism,Oxidative stress,NOS,Dopamine,Amino Acids,GTP

Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown.