Anti-GTF2F2 Antibody (4K636) is a Rabbit antibody targeting GTF2F2. Anti-GTF2F2 Antibody (4K636) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, IP, WB.

IgG

4K636

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with GTF2F2 monoclonal antibody, unconjugated (TMAB-06840) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-GTF2F2 antibody (TMAB-06840, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:50-200; IHC-Fr=1:100-200; IHC-P=1:100-200; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human GTF2F2

Human

GTF2F2

General transcription factor IIF subunit 2

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.