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Anti-GST Tag Antibody (3S373)
还可以产品编号 TMAY-01756
Anti-GST Tag Antibody (3S373) 是一种 Mouse 抗体，靶向 GST Tag。Anti-GST Tag Antibody (3S373) 可用于 WB,IP。
Anti-GST Tag Antibody (3S373)
Anti-GST Tag Antibody (3S373)
还可以产品编号 TMAY-01756
Anti-GST Tag Antibody (3S373) 是一种 Mouse 抗体，靶向 GST Tag。Anti-GST Tag Antibody (3S373) 可用于 WB,IP。
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抗原信息
| 产品描述 | Anti-GST Tag Antibody (3S373) is a Mouse antibody targeting GST Tag. Anti-GST Tag Antibody (3S373) can be used in WB,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 3S373 |
| 交叉反应 | Schistosoma japonicum |
| 验证活性 | 1. Anti-GST Tag was immunoprecipitated using: -Lane A: GST-ARG1-Flag transfected 293 cell lysate (0.5mg) -Lane B: Flag-ARG1-GST transfected 293 cell lysate (0.5mg) -2 μg anti-HA Tag mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-GST Tag rabbit monoclonal antibody, at 1:100 dilutionSecondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 2. Recognize GST tag fusion protein human ERK2.Anti-GST Tag mouse monoclonal antibody at 1:4000 -Lane A: GST-ERK2 (Recombinant protein) (100ng) -Lane B: GST-ERK2 (Recombinant protein) (50ng) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 3. Recognize GST tag fusion protein human USP7.Anti-GST Tag mouse monoclonal antibody at 1:4000 -Lane A: GST-USP7 (Recombinant protein) (100ng) -Lane B: GST-USP7 (Recombinant protein) (50ng) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 4. Recognize GST tag fusion proteins which expressed by 293 cell. -Anti-GST Tag mouse monoclonal antibody at 1:1250 or 1:2500 -Lane A: GST-FABP4-flag transfected 293 Cell Lysate (30ug) -Lane B: flag-FABP4-GST transfected 293 Cell Lysate (30ug) -Lane C: flag-AGR1-GST transfected 293 Cell Lysate (30ug) -Lane D: Negative control -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 5. Recognize GST tag fusion proteins which expressed by E. coli.Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250 -Lane A: GST-mBID transfected E.coli cell lysate (0.5ug) -Lane B: GST-mBID transfected E.coli cell lysate (0.2ug) -Lane C: GST-mBID transfected E.coli cell lysate (0.1ug) -Lane D: GST-mBID transfected E.coli cell lysate (0.05ug) -Lane E: GST-mBID transfected E.coli cell lysate (0.02ug) -Lane F: GST-YWHAB transfected E.coli cell lysate (0.5ug) -Lane G: GST-YWHAB transfected E.coli cell lysate (0.2ug) -Lane H: GST-YWHAB transfected E.coli cell lysate (0.1ug) -Lane I: GST-YWHAB transfected E.coli cell lysate (0.05ug) -Lane J: GST-YWHAB transfected E.coli cell lysate (0.02ug) -Lane K: Empty vecter (0.5ug) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 6. Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:1000 -Lane A: GST-mBID transfected E.coli cell lysate (0.1ug) -Lane B: GST-YWHAB transfected E.coli cell lysate (0.1ug) -Lane C: Empty vecter (0.1ug) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 7. Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:25000 -Lane A: GST-mBID transfected E.coli cell lysate (0.2ug) -Lane B: GST-YWHAB transfected E.coli cell lysate (0.2ug) -Lane C: Empty vecter (0.2ug) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:1000-1:10000; IP: 1-4 μl/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Glutathione S-transferase (GST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The glutathione S-transferase (GST) tag is another well-established affinity tag based on the strong affinity of GST for immobilized glutathione. The GST tag is best suitable for use in prokaryotic expression because GSTs are a family of multifunctional cytosolic proteins that are present in eukaryotic organisms but generally not found in bacteria. Similar to the MBP tag, GST tag has long been used to increase the solubility of fusion proteins in E. coli. GST-tagged proteins are captured by immobilized glutathione and then are eluted under mild, nondenaturing conditions using reduced glutathione. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant GST protein |
| 抗原种属 | Schistosoma japonicum |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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