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Anti-GRB2 Antibody (9L258)
还可以产品编号 TMAH-00511
别名 SH2/SH3 Adapter GRB2, Protein Ash, Growth Factor Receptor-Bound Protein 2, GRB2, ASH, Adapter Protein GRB2
Anti-GRB2 Antibody (9L258) 是一种抗体，靶向 GRB2。Anti-GRB2 Antibody (9L258) 可用于 ELISA, WB, IHC。
Anti-GRB2 Antibody (9L258)
Anti-GRB2 Antibody (9L258)
还可以产品编号 TMAH-00511 别名 SH2/SH3 Adapter GRB2, Protein Ash, Growth Factor Receptor-Bound Protein 2, GRB2, ASH, Adapter Protein GRB2
Anti-GRB2 Antibody (9L258) 是一种抗体，靶向 GRB2。Anti-GRB2 Antibody (9L258) 可用于 ELISA, WB, IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-GRB2 Antibody (9L258) is an antibody targeting GRB2. Anti-GRB2 Antibody (9L258) can be used in ELISA, WB, IHC. |
| 别名 | SH2/SH3 Adapter GRB2, Protein Ash, Growth Factor Receptor-Bound Protein 2, GRB2, ASH, Adapter Protein GRB2 |
| Ig Type | Rabbit IgG |
| 克隆号 | 9L258 |
| 交叉反应 | Human, Mouse, Rat |
| 验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate -All lanes: GRB2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 26, 21 kDa -Observed band size: 28 kDa 2. IHC image of TMAH-00511 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| 应用 | ELISA, WB, IHC |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Cytoplasm. Endosome. Golgi apparatus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human GRB2 |
| 抗原种属 | Human |
| 基因ID | 2885 |
| Uniprot ID | |
| 研究领域 | Cancer, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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