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Anti-GPR176 Polyclonal Antibody

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货号 TMAB-06701

Anti-GPR176 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 GPR176。Anti-GPR176 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-GPR176 Polyclonal Antibody

Anti-GPR176 Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-06701

Anti-GPR176 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 GPR176。Anti-GPR176 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍


生物活性
产品描述
Anti-GPR176 Polyclonal Antibody is a Rabbit antibody targeting GPR176. Anti-GPR176 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
反应种属
Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit,Sheep)
验证活性
1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GPR176 Polyclonal Antibody, Unconjugated (TMAB-06701) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Heart tissue lysates
Primary: Anti-GPR176 (TMAB-06701) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 53 kDa
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Multi-pass membrane protein.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景GPR176 is a Members of the G protein-coupled receptor family, such as GPR176, are cell surface receptors involved in responses to hormones, growth factors, and neurotransmitters (Hata et al., 1995 [PubMed 7893747]).[supplied by OMIM, Jul 2008].
抗原信息
免疫原
KLH conjugated synthetic peptide: human GPR176
抗原种属
Human
基因名称
GPR176
基因ID
蛋白名称
G-protein coupled receptor 176
Uniprot ID
功能
Orphan receptor.
化学信息
分子量Theoretical: 57 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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