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Anti-GPNMB Antibody (2N38)

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货号 TMAB-06676

Anti-GPNMB Antibody (2N38) 是一种 Mouse 抗体，靶向 GPNMB。Anti-GPNMB Antibody (2N38) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。

Anti-GPNMB Antibody (2N38)

Anti-GPNMB Antibody (2N38)

一键复制产品信息
Rating icon 还可以
货号 TMAB-06676

Anti-GPNMB Antibody (2N38) 是一种 Mouse 抗体，靶向 GPNMB。Anti-GPNMB Antibody (2N38) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。

规格价格库存数量
25 μL
¥ 780
5日内发货
50 μL
¥ 1,385
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-GPNMB Antibody (2N38) is a Mouse antibody targeting GPNMB. Anti-GPNMB Antibody (2N38) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG1, Kappa
克隆号
2N38
反应种属
Human,Rat
验证活性
1. Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-06676
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, A375, PC-12, THP-1
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 64-70 kDa
Observed MW: 70 kDa
3. Tissue: Human liver
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-06676
应用WBIHC-PIHC-FrICC/IFIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
组织特异性Not restricted to the melanocytic lineage.
构建方式Recombinant Antibody
纯化方式Protein G purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
抗原信息
基因名称
GPNMB
基因ID
10457
蛋白名称
Transmembrane glycoprotein NMB
功能
Could be a melanogenic enzyme.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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