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Anti-GLUT2 Antibody (4Y169)

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货号 TMAB-06550

Anti-GLUT2 Antibody (4Y169) 是一种 Rabbit 抗体,靶向 GLUT2。Anti-GLUT2 Antibody (4Y169) 可用于 WB,FCM。

Anti-GLUT2 Antibody (4Y169)

Anti-GLUT2 Antibody (4Y169)

一键复制产品信息
Rating icon 还可以
货号 TMAB-06550

Anti-GLUT2 Antibody (4Y169) 是一种 Rabbit 抗体,靶向 GLUT2。Anti-GLUT2 Antibody (4Y169) 可用于 WB,FCM。

规格价格库存数量
25 μL
¥ 780
5日内发货
50 μL
¥ 1,395
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍


生物活性
产品描述
Anti-GLUT2 Antibody (4Y169) is a Rabbit antibody targeting GLUT2. Anti-GLUT2 Antibody (4Y169) can be used in WB,FCM.
Ig Type
IgG
克隆号
4Y169
反应种属
Human,Mouse,Rat
验证活性
1. 25 μg total protein per lane of various lysates (see on figure) probed with GLUT2 monoclonal antibody, unconjugated (TMAB-06550) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 25 μg total protein per lane of various lysates (see on figure) probed with GLUT2 monoclonal antibody, unconjugated (TMAB-06550) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-GLUT2 antibody (TMAB-06550): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
应用WBFCM
推荐剂量
WB: 1:500-2000; FCM: 1μg/Test
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Membrane; Multi-pass membrane protein.
组织特异性Liver, insulin-producing beta cell, small intestine and kidney.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
研究背景Facilitative hexose transporter that mediates the transport of glucose, fructose and galactose. Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell.
抗原信息
免疫原
A synthesized peptide: human GLUT2
抗原种属
Human
基因名称
SLC2A2
基因ID
蛋白名称
Solute carrier family 2, facilitated glucose transporter member 2
Uniprot ID
功能
Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
化学信息
分子量Theoretical: 57 kDa. Actual: 57 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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