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Anti-GLUT2 Antibody (4Y169) 是一种 Rabbit 抗体,靶向 GLUT2。Anti-GLUT2 Antibody (4Y169) 可用于 WB,FCM。
Anti-GLUT2 Antibody (4Y169) 是一种 Rabbit 抗体,靶向 GLUT2。Anti-GLUT2 Antibody (4Y169) 可用于 WB,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 780 | 5日内发货 | |
| 50 μL | ¥ 1,395 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
| 产品描述 | Anti-GLUT2 Antibody (4Y169) is a Rabbit antibody targeting GLUT2. Anti-GLUT2 Antibody (4Y169) can be used in WB,FCM. |
| Ig Type | IgG |
| 克隆号 | 4Y169 |
| 反应种属 | Human,Mouse,Rat |
| 验证活性 | 1. 25 μg total protein per lane of various lysates (see on figure) probed with GLUT2 monoclonal antibody, unconjugated (TMAB-06550) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. 25 μg total protein per lane of various lysates (see on figure) probed with GLUT2 monoclonal antibody, unconjugated (TMAB-06550) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 3. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-GLUT2 antibody (TMAB-06550): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| 应用 | WBFCM |
| 推荐剂量 | WB: 1:500-2000; FCM: 1μg/Test |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. |
| 组织特异性 | Liver, insulin-producing beta cell, small intestine and kidney. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究背景 | Facilitative hexose transporter that mediates the transport of glucose, fructose and galactose. Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. |
| 免疫原 | A synthesized peptide: human GLUT2 |
| 抗原种属 | Human |
| 基因名称 | SLC2A2 |
| 基因ID | |
| 蛋白名称 | Solute carrier family 2, facilitated glucose transporter member 2 |
| Uniprot ID | |
| 功能 | Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. |
| 分子量 | Theoretical: 57 kDa. Actual: 57 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |