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顶部生物活性储存和溶解信息计算器

Anti-GLUL Polyclonal Antibody

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Anti-GLUL Polyclonal Antibody
产品编号 TMAB-00780

Anti-GLUL Polyclonal Antibody 是一种 Rabbit 抗体，靶向 GLUL。Anti-GLUL Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。

Anti-GLUL Polyclonal Antibody

Anti-GLUL Polyclonal Antibody

Rating icon 还可以
Anti-GLUL Polyclonal Antibody
产品编号 TMAB-00780

Anti-GLUL Polyclonal Antibody 是一种 Rabbit 抗体，靶向 GLUL。Anti-GLUL Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍

生物活性
产品描述
Anti-GLUL Polyclonal Antibody is a Rabbit antibody targeting GLUL. Anti-GLUL Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Ig Type
IgG
交叉反应
Mouse,Rat (predicted:Human,Pig,Cow,Sheep)
验证活性
1. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Glutamine synthetase Polyclonal Antibody, Unconjugated (TMAB-00780) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Raji Cell (Human) Lysate at 40 μg
Primary: Anti-Glutamine synthetase (TMAB-00780) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
应用
WB
IHC-P
IHC-Fr
IF
ELISA
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Mitochondrion.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 42 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.
抗原信息
免疫原
KLH conjugated synthetic peptide: human Glutamine synthetase
抗原种属
Human
基因ID
2752
Uniprot ID
P15104
研究领域
Alzheimer's disease,Glutamate
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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