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Anti-GLS1 Polyclonal Antibody
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产品编号 TMAB-00779
Anti-GLS1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 GLS1。Anti-GLS1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
Anti-GLS1 Polyclonal Antibody
Anti-GLS1 Polyclonal Antibody
还可以产品编号 TMAB-00779
Anti-GLS1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 GLS1。Anti-GLS1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-GLS1 Polyclonal Antibody is a Rabbit antibody targeting GLS1. Anti-GLS1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Cow) |
| 验证活性 | 1. Sample: Lane 1: Human U251 cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-GLS1 (TMAB-00779) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 75 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLS1) Polyclonal Antibody, Unconjugated (TMAB-00779) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLS1) Polyclonal Antibody, Unconjugated (TMAB-00779) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Isoform 1: Cytoplasm, cytosol. Isoform 3: Mitochondrion. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]. |
| 免疫原 | KLH conjugated synthetic peptide: human GLS1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Amino acid metabolism,Glutamate,Amino Acids |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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