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顶部生物活性储存和溶解信息计算器

Anti-GLP1R Polyclonal Antibody

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Anti-GLP1R Polyclonal Antibody
产品编号 TMAB-00777

Anti-GLP1R Polyclonal Antibody 是一种 Rabbit 抗体，靶向 GLP1R。Anti-GLP1R Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-GLP1R Polyclonal Antibody

Anti-GLP1R Polyclonal Antibody

Rating icon 还可以
Anti-GLP1R Polyclonal Antibody
产品编号 TMAB-00777

Anti-GLP1R Polyclonal Antibody 是一种 Rabbit 抗体，靶向 GLP1R。Anti-GLP1R Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍

生物活性
产品描述
Anti-GLP1R Polyclonal Antibody is a Rabbit antibody targeting GLP1R. Anti-GLP1R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Sample:
Lane 1: Mouse Pancreas tissue lysates
Lane 2: Mouse Liver tissue lysates (negative control)
Primary: Anti-GLP-1R (TMAB-00777) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa
2. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (TMAB-00777) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (TMAB-00777) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (TMAB-00777) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (TMAB-00777) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (TMAB-00777) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (TMAB-00777) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
应用
WB
IHC-P
IHC-Fr
IF
推荐剂量
WB: 1:500-2000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Multi-pass membrane protein.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景GLP1R is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.It has been suggested that this protein influences the feelings of satiety or hunger, sensation of glucose levels, control of glucagon sensitivity of islets, and non insulin-dependent diabetes mellitus. GLP1R is believed to be expressed in human pancreas,lung, brain, stomach, kidney and heart. ESTs have been isolated from skin and kidney libraries.
抗原信息
免疫原
KLH conjugated synthetic peptide: rat GLP-1R
抗原种属
Rat
基因ID
25051
Uniprot ID
P32301
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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