Anti-GLIS3 Polyclonal Antibody is a Rabbit antibody targeting GLIS3. Anti-GLIS3 Polyclonal Antibody can be used in ELISA,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat,Sheep

1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLIS3 Polyclonal Antibody, Unconjugated (TMAB-00776) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLIS3 Polyclonal Antibody, Unconjugated (TMAB-00776) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLIS3 Polyclonal Antibody, Unconjugated (TMAB-00776) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Tissue/cell: Sheep thyroid tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLIS3 Polyclonal Antibody, Unconjugated (TMAB-00776) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLIS3 Polyclonal Antibody, Unconjugated (TMAB-00776) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
6. Sample:
Lane 1: Human U251 cell lysates
Lane 2: Human U87MG cell lysates
Lane 3: Human A549 cell lysates
Lane 4: Human 293T cell lysates
Lane 5: Human MDA-MB-231 cell lysates
Primary: Anti-GLIS3 (TMAB-00776) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human GLIS3

Human

GLIS3

Zinc finger protein GLIS3

Krueppel like factor,Zinc Finger,Krueppel like factor,Pol II Transcription,Transcription Factors

GLIS3 is a member of the GLI similar zinc finger protein family, and encodes a nuclear protein with five C2H2 type zinc finger domains. It functions as both an activator and repressor of transcription, and is specifically involved in the development of pancreatic beta cells, thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full length nature of only two have been determined.