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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-GLI4 Polyclonal Antibody

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货号 TMAB-06505

Anti-GLI4 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 GLI4。Anti-GLI4 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-GLI4 Polyclonal Antibody

Anti-GLI4 Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-06505

Anti-GLI4 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 GLI4。Anti-GLI4 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-GLI4 Polyclonal Antibody is a Rabbit antibody targeting GLI4. Anti-GLI4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
反应种属
Human (predicted:Mouse,Rat,Pig,Cow,Horse)
验证活性
1. Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GLI4 Polyclonal Antibody, Unconjugated (TMAB-06505) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: A549 Cell (Human) Lysate at 30 μg
Primary: Anti-GLI4 (TMAB-06505) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Nucleus.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. GLI-4, also known as HKR4, is a 376 amino acid protein that localizes to the nucleus and contains seven C2H2-type zinc fingers. Belonging to the krueppel C2H2-type zinc-finger protein family, GLI-4 may function as a transcriptional regulator, effectively activating or repressing the transcription of target genes. The gene encoding GLI-4 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies.
抗原信息
免疫原
KLH conjugated synthetic peptide: human GLI4
抗原种属
Human
基因名称
GLI4
基因ID
2738
蛋白名称
Zinc finger protein GLI4
Uniprot ID
P10075
化学信息
分子量Theoretical: 41 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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