Anti-GJA5 Polyclonal Antibody is a Rabbit antibody targeting GJA5. Anti-GJA5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse

1. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Connexin-40 Polyclonal Antibody, Unconjugated (TMAB-00774) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Connexin-40 Polyclonal Antibody, Unconjugated (TMAB-00774) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Connexin-40 Polyclonal Antibody, Unconjugated (TMAB-00774) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. 25 μg total protein per Lane of various lysates probed with Connexin-40 polyclonal antibody, unconjugated (TMAB-00774) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Connexin-40

Human

GJA5

Gap junction alpha-5 protein

Cardiac arrhythmias,Endothelium,Gap Junctions

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.