Anti-GFP Antibody (1V694)

Name: Anti-GFP Antibody (1V694)
Brand: TargetMol
SKU: TMAY-02488
Rating: 4.5 (1 reviews)

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产品编号 TMAY-02488

别名 GFP Protein

Anti-GFP Antibody (1V694) 是一种 Rabbit 抗体，靶向 GFP。Anti-GFP Antibody (1V694) 可用于 WB,ELISA,IP。

Anti-GFP Antibody (1V694)

还可以

产品编号 TMAY-02488 别名 GFP Protein

Anti-GFP Antibody (1V694) 是一种 Rabbit 抗体，靶向 GFP。Anti-GFP Antibody (1V694) 可用于 WB,ELISA,IP。

规格	价格	库存
20 μL	¥ 790	5日内发货
100 μL	¥ 1,585	5日内发货
500 μL	¥ 3,990	5日内发货

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生物活性

产品描述	Anti-GFP Antibody (1V694) is a Rabbit antibody targeting GFP. Anti-GFP Antibody (1V694) can be used in WB,ELISA,IP.
别名	GFP Protein
Ig Type	Rabbit IgG
克隆号	1V694
交叉反应	Aequorea victoria
验证活性	1. Anti-GFP Tag was immunoprecipitated using: -Lane A: GFP transfected E.coli cell lysate (0.5mg) -Lane B: GFP transfected 293 cell lysate (0.5mg) -Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 2. Anti-GFP rabbit monoclonal antibody at 1:500 dilution. -Lane A: GFP transfected E.coli lysate (5ug) -Lane B: GFP transfected 293 Cell lysate (30ug) -Lane C: Non-transfected 293 Cells lysate (30ug) -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:27 kDa. -Observed band size:27 kDa. 3. Anti-GFP rabbit monoclonal antibody at 1:1000 dilution. -Sample: Recombinant Protein 10 ng -Lane 1: Aequorea victoria GFP Protein (His Tag) -Lane 2: EGFP / Enhanced Green Fluorescent Protein (His Tag) -Lane 3: Aequorea victoria Green fluorescent protein /enhanced GFP GFPSparkTM -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. 4. GFP was immunoprecipitated using: -Lane A:0.5 mg pCMV3-untagged Negative Control Vector -Lane B:0.5 mg pCMV3-C-GFPSpark Vector -Lane C:0.5 mg pCMV3-N-GFPSpark Vector -4 µL anti- GFP rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti- GFP rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions.
应用	WB ELISA IP
推荐剂量	WB: 1:1000-1:5000; ELISA: 1:10000-1:20000; IP: 1-5 μl/mg of lysate
抗体种类	Monoclonal
宿主来源	Rabbit
构建方式	This antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP (TMPY-02451; AAB65663; Ser2-Lys238).
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS
研究背景	Green fluorescent protein (GFP tag) is a ~27 kDa protein consisting of 238 amino acids derived from jellyfish Aequorea Victoria. GFP exhibits bright green fluorescent light when exposed to blue light. It has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms. Anti GFP-tag antibodies are often used in biological research and related fields. GFP tag antibodies are also often been used to study the expression of proteins and the interaction between proteins and proteins.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	Recombinant Protein: Aequorea victoria GFP protein (TMPY-02451)
抗原种属	Aequorea victoria

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.