Anti-GFAP Antibody (5E511) is a Rabbit antibody targeting GFAP. Anti-GFAP Antibody (5E511) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

5E511

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
9. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
10. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Polyclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
12. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Polyclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
13. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Polyclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
14. 25 μg total protein per lane of various lysates (see on figure) probed with GFAP monoclonal antibody, unconjugated (TMAB-06444) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
15. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose Red), DAPI (blue) was used to stain the cell nuclei.
16. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose Red), DAPI (blue) was used to stain the cell nuclei.
17. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose Red), DAPI (blue) was used to stain the cell nuclei.
18. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (orange), DAPI (blue) was used to stain the cell nuclei.
19. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (orange), DAPI (blue) was used to stain the cell nuclei.
20. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (TMAB-06444) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (orange), DAPI (blue) was used to stain the cell nuclei.

WB: 1:1000-5000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000

Monoclonal

Recombinant Protein: human GFAP protein

Human

GFAP

Glial fibrillary acidic protein

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.