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Anti-GFAP Antibody (3A643)
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产品编号 TMAB-00763
Anti-GFAP Antibody (3A643) 是一种 Rabbit 抗体，靶向 GFAP。Anti-GFAP Antibody (3A643) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-GFAP Antibody (3A643)
Anti-GFAP Antibody (3A643)
还可以产品编号 TMAB-00763
Anti-GFAP Antibody (3A643) 是一种 Rabbit 抗体，靶向 GFAP。Anti-GFAP Antibody (3A643) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 795 | 5日内发货 | |
| 50 μL | ¥ 1,390 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-GFAP Antibody (3A643) is a Rabbit antibody targeting GFAP. Anti-GFAP Antibody (3A643) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 3A643 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated (TMAB-00763) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 11. 25 μg total protein per Lane of various lysates probed with GFAP monoclonal antibody, unconjugated (TMAB-00763) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1:20000; IHC-P: 1:100-1:500; IHC-Fr: 1:100-1:500; IF: 1:100-1:500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm. Note=Associated with intermediate filaments. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| 免疫原 | A synthesized peptide: human GFAP |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Intermediate Filaments,Astrocyte marker,GFAP,Astrocyte,Glial |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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