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Anti-GDF-3 Antibody (5D320)
还可以产品编号 TMAY-00129
Anti-GDF-3 Antibody (5D320) 是一种 Rabbit 抗体，靶向 GDF-3。Anti-GDF-3 Antibody (5D320) 可用于 IHC-P,ICC/IF。
Anti-GDF-3 Antibody (5D320)
Anti-GDF-3 Antibody (5D320)
还可以产品编号 TMAY-00129
Anti-GDF-3 Antibody (5D320) 是一种 Rabbit 抗体，靶向 GDF-3。Anti-GDF-3 Antibody (5D320) 可用于 IHC-P,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-GDF-3 Antibody (5D320) is a Rabbit antibody targeting GDF-3. Anti-GDF-3 Antibody (5D320) can be used in IHC-P,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5D320 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunochemical staining of human GDF3 in human testis with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human GDF3 in human breast carcinoma with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). 3. Immunofluorescence staining of GDF3 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GDF3 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
| 应用 | IHC-P,ICC/IF |
| 推荐剂量 | IHC-P: 1:50-1:200; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human GDF-3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The GDF3 gene plays a fundamental role in embryonic morphogenesis. GDF3 expression is significantly decreased in human breast cancer tissues, and reconstitution of GDF3 in breast cancer may be a potential therapeutic approach to inhibit aggressive growth of breast cancer. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the center region of the Human GDF-3 |
| 抗原种属 | Human |
| 研究领域 | Early Mesodermal Lineage Markers |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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