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Anti-Gbx1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Gbx1。Anti-Gbx1 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF,FCM。
Anti-Gbx1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Gbx1。Anti-Gbx1 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
| 产品描述 | Anti-Gbx1 Polyclonal Antibody is a Rabbit antibody targeting Gbx1. Anti-Gbx1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| 反应种属 | Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Rabbit,Sheep,Fruit Fly,Chimpanzee) |
| 验证活性 | 1. Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Gbx1 antibody (TMAB-06368), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Gbx1) Polyclonal Antibody, Unconjugated (TMAB-06368) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| 应用 | IHC-PIHC-FrIFFCM |
| 推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | The homeobox is DNA sequence that is approximately 180 base pairs long that is involved in all steps of the developmental regulation of animals, fungi and plants, from embryogenesis to cell differentiation. The homeobox encodes a protein domain of about 60 amino acids, called the homeodomain, that can bind to DNA. The gastrulation brain homeobox 1 (GBX1) gene, along with another homeobox gene (EN2), maps to chromosome 7q36, and is expressed in septal and pallidal areas and in the basal forebrain cholinergic system, an area implicated in Alzheimer’s disease. The GBX1 gene encodes the GBX1 protein, which localizes to the nucleus and contains 1 homeobox DNA-binding domain. |
| 免疫原 | KLH conjugated synthetic peptide: human Gbx1 |
| 抗原种属 | Human |
| 基因名称 | GBX1 |
| 基因ID | |
| 蛋白名称 | Homeobox protein GBX-1 |
| Uniprot ID |
| 分子量 | Theoretical: 38 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |