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Anti-GAPDH Antibody (6G48)
还可以产品编号 TMAY-00247
Anti-GAPDH Antibody (6G48) 是一种 Rabbit 抗体，靶向 GAPDH。Anti-GAPDH Antibody (6G48) 可用于 WB,IP。
Anti-GAPDH Antibody (6G48)
Anti-GAPDH Antibody (6G48)
还可以产品编号 TMAY-00247
Anti-GAPDH Antibody (6G48) 是一种 Rabbit 抗体，靶向 GAPDH。Anti-GAPDH Antibody (6G48) 可用于 WB,IP。
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| 产品描述 | Anti-GAPDH Antibody (6G48) is a Rabbit antibody targeting GAPDH. Anti-GAPDH Antibody (6G48) can be used in WB,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 6G48 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-GAPDH rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: HepG2 Whole Cell Lysate. -Lane D: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:36 kDa. -Observed band size:36 kDa. 2. GAPDH was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg HepG2 Whole Cell Lysate. -Lane C:0.5 mg Jurkat Whole Cell Lysate. -4 µL anti-GAPDH rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-GAPDH rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 34 kDa. -Observed band size:36 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:2000; IP: IP:1-5 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human GAPDH (rh GAPDH; TMPY-02446; P04406; Met1-Glu335). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human GAPDH Protein (TMPY-02446) |
| 抗原种属 | Human |
| 研究领域 | Adaptor Proteins |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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