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Anti-GAPDH Antibody (5Z282)
还可以产品编号 TMAH-00474
别名 HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
Anti-GAPDH Antibody (5Z282) 是一种抗体，靶向 GAPDH。Anti-GAPDH Antibody (5Z282) 可用于 ELISA, WB, IHC, IF, FC。
Anti-GAPDH Antibody (5Z282)
Anti-GAPDH Antibody (5Z282)
还可以产品编号 TMAH-00474 别名 HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
Anti-GAPDH Antibody (5Z282) 是一种抗体，靶向 GAPDH。Anti-GAPDH Antibody (5Z282) 可用于 ELISA, WB, IHC, IF, FC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-GAPDH Antibody (5Z282) is an antibody targeting GAPDH. Anti-GAPDH Antibody (5Z282) can be used in ELISA, WB, IHC, IF, FC. |
| 别名 | HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD |
| Ig Type | Rabbit IgG |
| 克隆号 | 5Z282 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate,U87 whole cell lysate,JK whole cell lysate -All lanes: GAPDH antibody at 1:500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 36 kDa -Observed band size: 36 kDa 2. IHC image of TMAH-00474 diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 3. Immunofluorescence staining of HepG2 cell with TMAH-00474 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 4. Overlay Peak curve showing Hela cells surface stained with TMAH-00474 (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISA, WB, IHC, IF, FC |
| 推荐剂量 | WB:1:1000-1:5000; IHC:1:20-1:200; IF:1:20-1:200; FC:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| 研究背景 | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human GAPDH Protein |
| 抗原种属 | Human |
| 基因ID | 2597 |
| Uniprot ID | |
| 研究领域 | Isotype/Loading Controls, Neuroscience, Cancer, Metabolism, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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