Anti-Galectin 3 Polyclonal Antibody 3 is a Rabbit antibody targeting Galectin 3. Anti-Galectin 3 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat,Mouse

1. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
17. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
18. 25 μg total protein per lane of various lysates (see on figure) probed with Galectin 3 polyclonal antibody, unconjugated (TMAB-06290) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
19. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red), DAPI (blue) was used to stain the cell nuclei.
20. Paraformaldehyde-fixed, paraffin embedded Rat Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red), DAPI (blue) was used to stain the cell nuclei.
21. Paraformaldehyde-fixed, paraffin embedded Mouse Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red), DAPI (blue) was used to stain the cell nuclei.
22. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red), DAPI (blue) was used to stain the cell nuclei.
23. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Polyclonal Antibody, Unconjugated (TMAB-06290) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red), DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

Recombinant Protein: human Galectin-3 protein

Human

LGALS3

Galectin-3

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.