Anti-GABA Transporter 3 Polyclonal Antibody is a Rabbit antibody targeting GABA Transporter 3. Anti-GABA Transporter 3 Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat)

1. Sample: A431 (Human) Cell Lysate at 40 μg
Primary: Anti-GABA Transporter 3 (TMAB-06244) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 71 kD
2. Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-GABA Transporter 3 antibody (TMAB-06244)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human GABA Transporter 3/GAT 3

Human

SLC6A11

Sodium-and chloride-dependent GABA transporter 3

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.