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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-FUT4 Polyclonal Antibody 2

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货号 TMAB-06216

Anti-FUT4 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 FUT4。Anti-FUT4 Polyclonal Antibody 2 可用于 WB,FCM。

Anti-FUT4 Polyclonal Antibody 2

Anti-FUT4 Polyclonal Antibody 2

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Rating icon 还可以
货号 TMAB-06216

Anti-FUT4 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 FUT4。Anti-FUT4 Polyclonal Antibody 2 可用于 WB,FCM。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-FUT4 Polyclonal Antibody 2 is a Rabbit antibody targeting FUT4. Anti-FUT4 Polyclonal Antibody 2 can be used in WB,FCM.
Ig Type
IgG
反应种属
Human,Mouse
验证活性
1. Sample: Large intestine (Mouse) Lysate at 40 μg
Primary: Anti-FUT4 (TMAB-06216) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
2. Sample: LOVo (Human) Cell Lysate at 30 μg
Primary: Anti-FUT4 (TMAB-06216) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
3. Overlay histogram showing HL 60 cells stained with TMAB-06216 (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M P1 μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal, Orange line) (1 μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
应用WBFCM
推荐剂量
WB: 1:500-2000; FCM: 1μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
组织特异性Highest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq].
抗原信息
免疫原
KLH conjugated synthetic peptide: human FUT4
抗原种属
Human
基因名称
FUT4
基因ID
2526
蛋白名称
alpha-(1, 3)-fucosyltransferase 4
Uniprot ID
P22083
功能
May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
化学信息
分子量Theoretical: 58 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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