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Anti-FOXO3A Polyclonal Antibody 是一种 Rabbit 抗体,靶向 FOXO3A。Anti-FOXO3A Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
Anti-FOXO3A Polyclonal Antibody 是一种 Rabbit 抗体,靶向 FOXO3A。Anti-FOXO3A Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 |
产品描述 | Anti-FOXO3A Polyclonal Antibody is a Rabbit antibody targeting FOXO3A. Anti-FOXO3A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Rat rectum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FOXO3A) Polyclonal Antibody, Unconjugated (TMAB-00709) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Uterus tissue lysates Lane 3: Rat Testis tissue lysates Lane 4: Human MCF-7 cell lysates Lane 5: Human DU145 cell lysates Primary: Anti-FOXO3A (TMAB-00709) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 90 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors. Tissue Specificity : Ubiquitous. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
免疫原 | KLH conjugated synthetic peptide: human FOXO3A |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Forkhead Box,FOXO |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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