Anti-FOS Polyclonal Antibody 3 is a Rabbit antibody targeting FOS. Anti-FOS Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (TMAB-00700) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample: Large intestine (Mouse) Lysate at 40 μg Large intestine (Rat) Lysate at 40 μg
Primary: Anti-c-fos (TMAB-00700) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
3. Sample:
Brain (Mouse) Lysate at 40 μg
Brain (Rat) Lysate at 40 μg
Primary: Anti-c-fos (TMAB-00700) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human c-fos

Human

Transcription factors,Oncoproteins,Proto-oncogenes,Leucine Zipper,Soma marker