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Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625)

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货号 TMAB-06107

Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) 是一种 Rabbit 抗体,靶向 Formyl-Histone H2B type 2E (Lys109)。Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) 可用于 FCM, IF, IHC-Fr, IHC-P, WB。

Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625)

Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625)

一键复制产品信息
Rating icon 还可以
货号 TMAB-06107

Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) 是一种 Rabbit 抗体,靶向 Formyl-Histone H2B type 2E (Lys109)。Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) 可用于 FCM, IF, IHC-Fr, IHC-P, WB。

规格价格库存数量
25 μL
¥ 795
5日内发货
50 μL
¥ 1,390
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍


生物活性
产品描述
Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) is a Rabbit antibody targeting Formyl-Histone H2B type 2E (Lys109). Anti-Formyl-Histone H2B type 2E (Lys109) Antibody (6G625) can be used in FCM, IF, IHC-Fr, IHC-P, WB.
Ig Type
IgG
克隆号
6G625
反应种属
Human,Mouse,Rat
验证活性
1. 25 ug total protein per lane of various lysates (see on figure) probed with Formyl-Histone H2B type 2E (Lys109) monoclonal antibody, unconjugated (TMAB-06107) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Formyl-Histone H2B type 2E (Lys109) antibody (TMAB-06107, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
应用FCMIFIHC-FrIHC-PWB
推荐剂量
FCM=1:50-100; IF=1:100-200; IHC-Fr=1:100-200; IHC-P=1:100-200; WB=1:500-2000
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
研究背景Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
抗原信息
免疫原
A synthesized peptide: human Histone H2B type 2E around the formylation site of K109
抗原种属
Human
基因名称
H2BC21
基因ID
蛋白名称
Histone H2B type 2-E
Uniprot ID
化学信息
分子量Theoretical: 14 kDa. Actual: 14 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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