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Anti-Flag Tag Antibody (7J897)

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Anti-Flag Tag Antibody (7J897)
产品编号 TMAC-01502

Anti-Flag Tag Antibody (7J897) 是一种 Mouse 抗体，靶向 Flag Tag。Anti-Flag Tag Antibody (7J897) 可用于 WB,IF,IP。

Anti-Flag Tag Antibody (7J897)

Anti-Flag Tag Antibody (7J897)

Rating icon 还可以
Anti-Flag Tag Antibody (7J897)
产品编号 TMAC-01502

Anti-Flag Tag Antibody (7J897) 是一种 Mouse 抗体，靶向 Flag Tag。Anti-Flag Tag Antibody (7J897) 可用于 WB,IF,IP。

规格价格库存数量
50 μL
¥ 785
5日内发货
100 μL
¥ 1,185
5日内发货
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产品介绍

生物活性
产品描述
Anti-Flag Tag Antibody (7J897) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (7J897) can be used in WB,IF,IP.
交叉反应
Human,Mouse,Rat
验证活性
1. 1ug Flag fusion protein+ Primary antibody TMAC-01502 dilution at 1:10,000.
2. IF analysis of 293 cells transfected with a Flag-tag protein, using anti-Flag-Tag Mouse mAb TMAC-01502 at a 1:2000 dilution (blue DAPI, red anti-Flag).
3. IP antibody use 5ug Flag Mouse IgG1 per ml Lysate WB 1:5000, 1 untransfected 293 cell lysate, 2 transfected 293 cell lysate with Flag-tag fusion protein, 3 IP (transfected 293+ normal Mouse IgG+Protein G agarose, 4 IP (transfected 293+anti- Flag mAb+ Protein G agarose), 5 IP (transfected 293+Protein G), 6 Recombinant protein (E.coli).
应用
WB
IF
IP
推荐剂量
WB: 1:5000-10000; IF: 1:2000; IP: 1:5000
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式Affinity purification using immunogen.
性状Liquid
缓冲液Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
偶联与修饰
偶联
Unconjugated
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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