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Anti-Flag Tag Antibody (2K280) 是一种 Mouse 抗体,靶向 Flag Tag。Anti-Flag Tag Antibody (2K280) 可用于 WB。
Anti-Flag Tag Antibody (2K280) 是一种 Mouse 抗体,靶向 Flag Tag。Anti-Flag Tag Antibody (2K280) 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 795 | 5日内发货 | |
100 μL | ¥ 1,180 | 5日内发货 |
产品描述 | Anti-Flag Tag Antibody (2K280) is a Mouse antibody targeting Flag Tag. Anti-Flag Tag Antibody (2K280) can be used in WB. |
验证活性 | 1. Western blot analysis of Recombinant protein, using TMAC-01503 diluted at 1) 1:5,000, 2) 1:10,000. |
应用 | |
推荐剂量 | WB: 1:5000-10000 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | Affinity purification using immunogen. |
性状 | Liquid |
缓冲液 | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
偶联 | Unconjugated |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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