Anti-Filaggrin Polyclonal Antibody is a Rabbit antibody targeting Filaggrin. Anti-Filaggrin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Filaggrin Polyclonal Antibody, Unconjugated (TMAB-00680) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Filaggrin Polyclonal Antibody, Unconjugated (TMAB-00680) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Filaggrin

Human

Epi / Endo-thelial,Cytokeratin,Cytokeratins