Anti-Fibulin 5 Polyclonal Antibody is a Rabbit antibody targeting Fibulin 5. Anti-Fibulin 5 Polyclonal Antibody can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

Human

1. Sample:
Lane 1: Recombinant human Fibulin 5 protein, C-His (Fibulin 5 (TMAB-06027) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 30 kDa
2. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Fibulin 5 Polyclonal Antibody, Unconjugated (TMAB-06027) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Fibulin 5 Polyclonal Antibody, Unconjugated (TMAB-06027) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Fibulin 5 Polyclonal Antibody, Unconjugated (TMAB-06027) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. 4% Paraformaldehyde-fixed WI-38 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Fibulin 5) polyclonal Antibody, unconjugated (TMAB-06027) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

ICC/IF=1:50-200; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Polyclonal

Recombinant Protein: human Fibulin 5 protein

Human

FBLN5

Fibulin-5

Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Could be a vascular ligand for integrin receptors and may play a role in vascular development and remodeling.