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Anti-FH/Fumarate Hydratase Antibody (5L839)
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产品编号 TMAY-02240
别名 MCUL1, MCL, LRCC, HLRCC, fumarate hydratase, FMRD
Anti-FH/Fumarate Hydratase Antibody (5L839) 是一种 Mouse 抗体，靶向 FH/Fumarate Hydratase。Anti-FH/Fumarate Hydratase Antibody (5L839) 可用于 WB,ELISA。
Anti-FH/Fumarate Hydratase Antibody (5L839)
Anti-FH/Fumarate Hydratase Antibody (5L839)
还可以产品编号 TMAY-02240 别名 MCUL1, MCL, LRCC, HLRCC, fumarate hydratase, FMRD
Anti-FH/Fumarate Hydratase Antibody (5L839) 是一种 Mouse 抗体，靶向 FH/Fumarate Hydratase。Anti-FH/Fumarate Hydratase Antibody (5L839) 可用于 WB,ELISA。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,694 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-FH/Fumarate Hydratase Antibody (5L839) is a Mouse antibody targeting FH/Fumarate Hydratase. Anti-FH/Fumarate Hydratase Antibody (5L839) can be used in WB,ELISA. |
| 别名 | MCUL1, MCL, LRCC, HLRCC, fumarate hydratase, FMRD |
| Ig Type | Mouse IgG1 |
| 克隆号 | 5L839 |
| 交叉反应 | Human |
| 验证活性 | Anti-FH mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:55 kDa. -Observed band size:47 kDa |
| 应用 | WB,ELISA |
| 推荐剂量 | WB: 1:500-1:1000; ELISA: 1:5000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Fumarate Hydratase / FH (rh Fumarate Hydratase / FH; TMPY-02003; P07954-1; Ala 45-Lys 510). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Fumarate Hydratase (FH) is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. Fumarate Hydratase is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. Individuals with hemizygous germline fumarate hydratase (FH) mutations are predisposed to renal cancer. These tumors predominantly exhibit functional inactivation of the remaining wild-type allele, implicating FH inactivation as a tumor-promoting event. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human Fumarate Hydratase / FH protein (TMPY-02003) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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