Anti-Ferredoxin Reductase Polyclonal Antibody is a Rabbit antibody targeting Ferredoxin Reductase. Anti-Ferredoxin Reductase Polyclonal Antibody can be used in FCM.

IgG

Mouse (predicted:Human,Rat,Horse)

Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-Ferredoxin Reductase antibody (TMAB-05964)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Ferredoxin Reductase

Human

FDXR

NADPH:adrenodoxin oxidoreductase, mitochondrial

Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.