Anti-FASN Polyclonal Antibody is a Rabbit antibody targeting FASN. Anti-FASN Polyclonal Antibody can be used in ELISA,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat (predicted:Mouse,Sheep)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-FASN Polyclonal Antibody, Unconjugated (TMAB-00659) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Primary: Anti-FASN (TMAB-00659) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 272 kDa
Observed band size: 250 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: humanFASN

Human

FASN

Fatty acid synthase

Integration of energy metabolism,Metabolism of lipids and lipoproteins,Enzymes,Synthesis,Energy Metabolism,Integration of energy,Lipid metabolism,Fatty acid oxidation,Cancer,Obesity,Metabolism,Energy Metabolism,Lipid metabolism

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.