Anti-FANCF Polyclonal Antibody is a Rabbit antibody targeting FANCF. Anti-FANCF Polyclonal Antibody can be used in FCM.

IgG

Mouse (predicted:Human,Rat,Cow)

Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-FANCF antibody (TMAB-05897)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488R
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human FANCF

Human

FANCF

Fanconi anemia group F protein

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability (By similarity).