Anti-FAK Polyclonal Antibody is a Rabbit antibody targeting FAK. Anti-FAK Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Tissue/cell: mouse lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-FAK/PTK2 Polyclonal Antibody, Unconjugated (TMAB-00652) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00652) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 3: Cerebrum (Rat) Lysate at 40 μg
Lane 4: 293T (Human) Cell Lysate at 30 μg
Lane 5: A431 (Human) Cell Lysate at 30 μg
Lane 6: Huvec (Human) Cell Lysate at 30 μg
Lane 7: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-FAK (TMAB-00652) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kDa
Observed band size: 120 kDa
4. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00652) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00652) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human FAK

Human

FAK family,Integrins,Focal Adhesions,FAK / PYK