Anti-factor XIII Polyclonal Antibody is a Rabbit antibody targeting factor XIII. Anti-factor XIII Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit)

1. Sample: Placenta (mouse) Cell Lysate at 40 μg
Primary: Anti-factor XIII (TMAB-05798) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76 kD
Observed band size: 76 kD
2. Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-factor XIII antibody (TMAB-05798)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human factor XIII

Human

F13B

Coagulation factor XIII B chain