Anti-Ezrin Antibody (7C964) is a Mouse antibody targeting Ezrin. Anti-Ezrin Antibody (7C964) can be used in WB,IP.

Monoclonal Mouse IgG1

7C964

Human

1. Anti-EZR mouse monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell Lysate.

-Lane B: Jurkat Whole Cell Lysate.

-Lane C: A431 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:69 kDa.

-Observed band size:90 kDa(We are unsure as to the identity of these extra bands.)

2. EZR was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-Lane B:0.5 mg HepG2 Whole Cell Lysate

-0.5 µL anti-EZR mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

-Primary antibody:

-Anti-EZR mouse monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 69 kDa.

-Observed band size: 90 kDa

WB,IP

WB: 1:500-1:1000; IP: 0.1-0.5 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Human Ezrin / villin 2 / EZR Protein (TMPY-03053)

Human