Anti-ETS1 Antibody (5E990) is a Rabbit antibody targeting ETS1. Anti-ETS1 Antibody (5E990) can be used in WB,FCM.

IgG

5E990

Human,Mouse

1. The Jurkat (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-ETS1 antibody (TMAB-05718): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 25 μg total protein per lane of various lysates (see on figure) probed with ETS1 monoclonal antibody, unconjugated (TMAB-05718) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; FCM: 1μg/Test

Monoclonal

A synthesized peptide: human ETS1

Human

ETS1

Protein C-ets-1

Transcription factor.