Anti-ERK5 Antibody (6Y531) is a Rabbit antibody targeting ERK5. Anti-ERK5 Antibody (6Y531) can be used in WB.

IgG

6Y531

Human,Mouse (predicted:Rat)

1. Sample:
Testis (Mouse) Lysate at 40 μg
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-ERK5 (TMAB-00638) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 110 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:2000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: MCF-7, 2: 293T, 3: NIH/3T3, 4: PC- 12
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 88 kDa
Observed MW: 115 kDa

WB: 1:500-2000

Monoclonal

Recombinant Protein: human ERK5 protein, around C-terminal 200aa

Human

MAPK7

Mitogen-activated protein kinase 7

Neural Signal Transduction,Kinases,MAPK Pathway,Other Kinases

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression.