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Anti-EphA2 Polyclonal Antibody
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产品编号 TMAB-00624
Anti-EphA2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 EphA2。Anti-EphA2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-EphA2 Polyclonal Antibody
Anti-EphA2 Polyclonal Antibody
还可以产品编号 TMAB-00624
Anti-EphA2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 EphA2。Anti-EphA2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-EphA2 Polyclonal Antibody is a Rabbit antibody targeting EphA2. Anti-EphA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Dog,Cow,Monkey) |
| 验证活性 | 1. Sample: NIH/3T3 (Mouse) Cell Lysate at 30 μg Primary: Anti-EphA2 (TMAB-00624) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 105 kDa Observed band size: 115 kDa 2. Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-EphA2/EpHreceptor A2 Polyclonal Antibody, Unconjugated (TMAB-00624) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Human colon carcinoma lysates Lysate at 30 μg Intestine (Mouse) Lysate at 30 μg Primary: Anti-EphA2/EpHreceptor A2 (TMAB-00624) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/3000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa, 52/55 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell junction, focal adhesion. Note=Present at regions of cell-cell contacts but also at the leading edge of migrating cells. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]. |
| 免疫原 | KLH conjugated synthetic peptide: human EphA2 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Eph Receptors,Growth Cone,Axonal Guidance Proteins,Kinases,Tyrosine Kinase Receptors |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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