Anti-Eotaxin/CCL11 Polyclonal Antibody is a Rabbit antibody targeting Eotaxin/CCL11. Anti-Eotaxin/CCL11 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.

IgG

Mouse (predicted:Human,Rat)

1. Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CCL11/Eotaxin 1 Polyclonal Antibody (TMAB-00619) at 1:100 for two hours at room temperature (green channel).
2. Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CCL11) Polyclonal Antibody, Unconjugated (TMAB-00619) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Eotaxin 1

Human

CCL11

Eotaxin

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.