Anti-eNOS Polyclonal Antibody 2 is a Rabbit antibody targeting eNOS. Anti-eNOS Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,GuineaPig)

1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (eNOS) Polyclonal Antibody, Unconjugated (TMAB-00617) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample:
K562 (Human) Cell Lysate at 40 μg
Primary: Anti-eNOS (TMAB-00617) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDa
3. Sample:
A431 Cell (Human) Lysate at 30 μg
Primary: Anti-eNOS (TMAB-00617) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDa
4. Sample:
K562Cell (Human) Lysate at 30 μg
Primary: Anti-eNOS (TMAB-00617) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human eNos

Human

Response to hypoxia,Platelets,Nitric oxide associated,Oxidative Stress,Hypoxia,Oxidative stress,NOS