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Anti-Endostatin Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Endostatin。Anti-Endostatin Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Endostatin Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Endostatin。Anti-Endostatin Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,175 | 5日内发货 | |
100 μL | ¥ 1,970 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-Endostatin Polyclonal Antibody is a Rabbit antibody targeting Endostatin. Anti-Endostatin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Mouse (predicted:Rat) |
验证活性 | 1. Sample: Liver (Mouse) Lysate at 40 μg Primary: Anti-Endostatin (TMAB-00610) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/190 kDa Observed band size: 155 kDa 2. Tissue/cell: human kidney carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Endostatin Polyclonal Antibody, Unconjugated (TMAB-00610) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted, extracellular space, extracellular matrix. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Two transcript variants encoding different isoforms have been found for this gene. |
免疫原 | KLH conjugated synthetic peptide: human Endostatin |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Angiogenic inhibitory factors,Inhibitors,Collagen |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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