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Anti-Endostatin Polyclonal Antibody

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Anti-Endostatin Polyclonal Antibody
产品编号 TMAB-00610

Anti-Endostatin Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Endostatin。Anti-Endostatin Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-Endostatin Polyclonal Antibody

Anti-Endostatin Polyclonal Antibody

Rating icon 还可以
Anti-Endostatin Polyclonal Antibody
产品编号 TMAB-00610

Anti-Endostatin Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Endostatin。Anti-Endostatin Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍

生物活性
产品描述
Anti-Endostatin Polyclonal Antibody is a Rabbit antibody targeting Endostatin. Anti-Endostatin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse (predicted:Rat)
验证活性
1. Sample:
Liver (Mouse) Lysate at 40 μg
Primary: Anti-Endostatin (TMAB-00610) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20/190 kDa
Observed band size: 155 kDa
2. Tissue/cell: human kidney carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Endostatin Polyclonal Antibody, Unconjugated (TMAB-00610) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted, extracellular space, extracellular matrix.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Two transcript variants encoding different isoforms have been found for this gene.
抗原信息
免疫原
KLH conjugated synthetic peptide: human Endostatin
抗原种属
Human
基因ID
Uniprot ID
研究领域
Angiogenic inhibitory factors,Inhibitors,Collagen
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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