Anti-Elastin Polyclonal Antibody is a Rabbit antibody targeting Elastin. Anti-Elastin Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Elastin) Polyclonal Antibody, Unconjugated (TMAB-00604) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Elastin Polyclonal Antibody, Unconjugated (TMAB-00604) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Elastin Polyclonal Antibody, Unconjugated (TMAB-00604) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Sample:
Lane 1: Lung (Mouse) Lysate at 40 μg
Lane 2: Liver (Mouse) Lysate at 40 μg
Lane 3: Uterus (Rat) Lysate at 40 μg
Lane 4: Liver (Rat) Lysate at 40 μg
Primary: Anti-Elastin (TMAB-00604) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-80 kDa
Observed band size: 75 kDa
5. Sample:
Lung (Mouse) Lysate at 40 μg
Primary: Anti-Elastin (TMAB-00604) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
6. Sample:
Uterus (Mouse) Lysate at 40 μg
Primary: Anti-Elastin (TMAB-00604) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Elastin

Human

ELN

Elastin

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.