Anti-EHD1 Antibody (5A616) is a Rabbit antibody targeting EHD1. Anti-EHD1 Antibody (5A616) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.

IgG

5A616

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with EHD1 monoclonal antibody, unconjugated (TMAB-05478) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-EHD1 antibody (TMAB-05478, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human EHD1

Human

EHD1

EH domain-containing protein 1

Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes.